Identifikasi Molekuler Trichoderma spp. Indigenous dari Rizosfer Beberapa Varietas Padi Asal Kabupaten Lima Puluh Kota dan Kota Payakumbuh

##plugins.themes.academic_pro.article.sidebar##

Published Jan 6, 2022
https://doi.org/10.55043/agroteknika.v5i1.114
Download
PDF
Statistic

Downloads

Download data is not yet available.
Vol 5 No 1 (2022): Juni 2022

##plugins.themes.academic_pro.article.main##

Nelson Elita
Politeknik Pertanian Negeri Payakumbuh
Eka Susila
Politeknik Pertanian Negeri Payakumbuh
Agustamar Agustamar
Politeknik Pertanian Negeri Payakumbuh
Rizki Rizki
Politeknik Pertanian Negeri Payakumbuh

Abstract

Peranan mikroba meningkatkan hasil padi metode SRI beragam dipengaruhi jenis,  kombinasi mikroorganisme, daya adaptasi, dan teknik aplikasi. Hasil penelitian menunjukkan aplikasi mikroba indigenous mampu meningkatkan hasil tanaman padi metode SRI. Jenis mikroba tanah jamur Trichoderma spp. umumnya banyak ditemukan merupakan jamur tanah biasanya ditemukan pada rizosfer tanaman, termasuk rizosfer tanaman padi.  Tujuan penelitian untuk mengetahui jenis Trichoderma spp. indigenous secara makroskopis dan molekuler asal rizosfer beberapa varietas padi di Kabupaten Lima Puluh Kota dan Kota Payakumbuh. Metode yang digunakan adalah eksplorasi, isolasi dan karakterisasi. Eksplorasi Trichoderma spp. indigenous diambil dari tanah rizosfer padi di Kabupaten Lima Puluh Kota dan Kota Payakumbuh. Isolasi dan karakterisasi jamur Trichoderma spp. indigenous dilakukan di laboratorium Mikrobiologi Politeknik Pertanian Negeri Payakumbuh dan teknik molekuler dengan Amplifikasi PCR di Balai Penelitian Tanaman Buah Tropika di Solok. Hasil penelitian diperoleh isolat T1-KK (Kuriak Kuning), T2-PW (Pandan Wangi), T3- J (Junjuang) ketiganya asal Kabupaten Lima Puluh Kota, T4- S (Sokan), T5-KM (Ketan Merah), T6-SB (Siliah Baganti) asal Kota Payakumbuh. Hasil amplifikasi PCR diperoleh jenis isolat Trichoderma spp dari masing-masing isolat tersebut adalah T1-KK jenis jamur Trichoderma asperellum, T2-PW jenis jamur Trichoderma harzianum dan Trichoderma asperellum, T3-J jenis jamur Trichoderma harzianum dan Trichoderma asperellum, T4-S jenis jamur Thichoderma asperellum, T5-KM jenis jamur Trichoderma harzianum dan Trichoderma asperellum, dan T6-SB jenis jamur Trichoderma harzianum dan Trichoderma asperellum.

##plugins.themes.academic_pro.article.details##

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Author Biographies

Nelson Elita, Politeknik Pertanian Negeri Payakumbuh

Program Budi Daya Tanaman Pangan

Eka Susila, Politeknik Pertanian Negeri Payakumbuh

Program Budi Daya Tanaman Pangan

Agustamar Agustamar, Politeknik Pertanian Negeri Payakumbuh

Program Budi Daya Tanaman Pangan

Rizki Rizki, Politeknik Pertanian Negeri Payakumbuh

Program Budi Daya Tanaman Pangan

How to Cite
Elita, N., Susila, E., Agustamar, A., & Rizki, R. (2022). Identifikasi Molekuler Trichoderma spp. Indigenous dari Rizosfer Beberapa Varietas Padi Asal Kabupaten Lima Puluh Kota dan Kota Payakumbuh. Agroteknika, 5(1), 1-13. https://doi.org/10.55043/agroteknika.v5i1.114

References

Badaluddin, N. A., Jamaluddin, S. N. T., Ihsam, N. S., Sajili, M. H., Khalit, S. I., & Mohamed, N. A. (2018). Molecular Identification of Isolated Fungi from Kelantan and Terengganu Using Internal Transcriber Spacer (ITS) Region. Journal of Agrobiotechnology, 9(1S), 222-231. Retrived from https://journal.unisza.edu.my/agrobiotechnology/index.php/agrobiotechnology/article/view/146
Bottacini, F., Morrissey, R., Esteban-Torres, M., James, K., van Breen, J., Dikareva, E., & van Sinderen, D. (2018). Comparative genomics and genotype-phenotype associations in Bifidobacterium breve. Scientific reports, 8(1), 1-14. https://doi.org/10.1038/s41598-018-28919-4
Cai, F., Chen, W., Wei, Z., Pang, G., Li, R., Ran, W., & Shen, Q. (2015). Colonization of Trichoderma harzianum strain SQR-T037 on tomato roots and its relationship to plant growth, nutrient availability and soil microflora. Plant and Soil, 388(1), 337-350. https://doi.org/10.1007/s11104-014-2326-z
Carvalhais, L. C., Dennis, P. G., Badri, D. V., Kidd, B. N., Vivanco, J. M., & Schenk, P. M. (2015). Linking jasmonic acid signaling, root exudates, and rhizosphere microbiomes. Molecular Plant-Microbe Interactions, 28(9), 1049-1058. https://doi.org/10.1094/MPMI-01-15-0016-R
Contreras-Cornejo, H. A., Macías-Rodríguez, L., Del-Val, E. K., & Larsen, J. (2016). Ecological functions of Trichoderma spp. and their secondary metabolites in the rhizosphere: interactions with plants. FEMS microbiology ecology, 92(4), fiw036. https://doi.org/10.1093/femsec/fiw036
Elita, N., & Agustamar, Y. (2013). Korelasi Mikroorganisme Pelarut Fosfat dan Pupuk P Dalam Pola SRI-Organik Untuk Meningkatkan Mutu Sawah Intensifikasi Serta Produksi Padi. Artikel Ilmiah. Jurnal Penelitian LUMBUNG, 12(1).
Elita, N., Harmailis, H., Erlinda, R., & Susila, E. (2021). Pengaruh Aplikasi Trichoderma spp. Indigenous terhadap Hasil Padi Varietas Junjuang Menggunakan System of Rice Intensification. Jurnal Tanah dan Iklim, 45(1), 79-89. http://dx.doi.org/10.21082/jti.v45n1.2021.79-89
Elita, N., Erlinda, R., & Agustamar, A. (2020). The Effect of Bioorganic Dosage with N, P Fertilizer on Rice Production of Sri Methods and Increased Nutrient Content of Paddy Soil Intensification. Journal of Applied Agricultural Science and Technology, 4(2), 155-169. https://doi.org/10.32530/jaast.v4i2.162
Elita, N., Susila, E., & Yefriwati. (2018). The potential types of indigenous arbuscular mycorrhizal fungi as sources of inoculum and their effect on rice production using the system of rice intensification method. Pakistan Journal of Nutrition, 17(12), 696-701. https://doi.org/10.3923/pjn.2018.696.701
Erlinda, R., Elita, N., & Agustamar (2020). The Effect Of Indigenous Azotobacter Isolate On Rice Results Of Sri And Land Quality Methods. International Journal of Advanced Research, 8(1), 185-193. http://dx.doi.org/10.21474/IJAR01/10281
Erlinda, R., Elita, N., & Susiawan, E. (2019). Pemanfaatan pupuk bioorganik plus untuk meningkatkan produksi padi metode SRI. Journal of Applied Agricultural Science and Technology, 3(1), 57-66. https://doi.org/10.32530/jaast.v3i1.93
Ghaffari, M. R., Ghabooli, M., Khatabi, B., Hajirezaei, M. R., Schweizer, P., & Salekdeh, G. H. (2016). Metabolic and transcriptional response of central metabolism affected by root endophytic fungus Piriformospora indica under salinity in barley. Plant molecular biology, 90(6), 699-717. https://doi.org/10.1007/s11103-016-0461-z.
Pandian, R.T.P., Raja, M., Kumar, A., Sharma, P.R., 2016. Morphological and molecular characterization of Trichoderma asperellum strain Ta13. Indian. Phytopathol. 69, 297–303. https://www.semanticscholar.org/paper/Morphological-and-molecular-characterization-of-Pandian-Raja/5029b79af371d18326f80571923a672a5b2caf67.
Watanabe, T. (2010). Pictorial Atlas of Soil and Seed Fungi. Morphologies of Cultured Fungi and Key to Species, Third Edition. 3rd Edition. CRC Press LCC. New York, Wahington, D.C. ISBN 9781439804193. Published May 26, 2010 by CRC Press. 426 Pages 2428 B/W Illustrations
Waghunde, R. R., Shelake, R. M., & Sabalpara, A. N. (2016). Trichoderma: A significant fungus for agriculture and environment. African journal of agricultural research, 11(22), 1952-1965. https://doi.org/10.5897/AJAR2015.10584.
Zeilinger, S., Gruber, S., Bansal, R., & Mukherjee, P. K. (2016). Secondary metabolism in Trichoderma–chemistry meets genomics. Fungal biology reviews, 30(2), 74-90. https://doi.org/10.1016/j.fbr.2016.05.001
Zin, N. A., & Badaluddin, N. A. (2020). Biological functions of Trichoderma spp. for agriculture applications. Annals of Agricultural Sciences, 65(2), 168-178. https://doi.org/10.1016/j.aoas.2020.09.003
Zhu, Z. X., & Zhuang, W. Y. (2015). Trichoderma (Hypocrea) species with green ascospores from China. Persoonia: Molecular Phylogeny and Evolution of Fungi, 34, 113. https://doi.org/10.3767/003158515X686732