Struktur Genetik Molekuler Selada Laut (Ulva lactuca) di Pantai Ulee Lheue, Indonesia
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Abstract
Pantai Ulee Lheue merupakan kawasan pantai di Kota Banda Aceh yang dikembangkan untuk wisata. Spesies rumput laut makro alga tersebar luas di Pantai Ulee Lheue namun belum pernah dimanfaatkan. Satu diantara selada laut hijau (sea lettuce) yang melimpah di Pantai Ulee Lheue yaitu Ulva lactuca. Penelitian ini bertujuan untuk mengidentifikasi struktur genetik Ulva lactuca yang berasal dari Pantai Ulee Lheue, Aceh, Indonesia secara molekuler. Metode yang digunakan pada penelitian ini yaitu skrining morfologi Ulva lactuca yang berpedoman pada literatur jurnal kemudian analisis molekuler ekstraksi DNA genom menggunakan gen primer ITS. Selanjutnya pengujian jarak genetik dengan metode Trichotomy melalui software Treeview X dari analisis statistik GenAlex. Hasil penelitian menunjukkan nilai Fit tidak ada perbedaan yang signifikan dengan Fis. Nilai total migran (Nm) menunjukkan nilai yang berbeda yaitu pada lokus ITS1 0,330 dan ITS 2 yaitu 0,094. Variasi alel lokus ITS dibedakan dengan jelas dari nilai PIC >0,5 artinya memiliki nilai polimorfis yang tinggi. Panjang alel dalam PCR teramplifikasi 300 bp s/d 500 bp. Identifikasi frekuensi statistik gen ITS dari sampel diperoleh pada kedua lokus menunjukkan nilai yang sama (1,000), Fst menunjukkan nilai dalam lokus ITS 1 adalah 0,431, dan ITS 2 0,726. Kekerabatan sangat erat diperoleh dari analisis filogenetik terbagi dalam dua (2) kelompok. Sampel UL8 terpisah dengan sampel UL1, UL2, UL3, UL4, UL5, UL6, UL7, UL9, UL10, UL11, UL12, UL13, UL14 dan UL15. Namun sampel UL10 dan UL14 berada dalam 1 klaster sama dengan sampel UL9 dan UL13. Kekerabatan tersebut kemungkinan dikarenakan spesies Ulva berasal dari pesisir pantai yang sama.
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How to Cite
Hayati, R., Rahly, F., & Majid, M. I. (2023). Struktur Genetik Molekuler Selada Laut (Ulva lactuca) di Pantai Ulee Lheue, Indonesia. Agroteknika, 6(2), 249-261. https://doi.org/10.55043/agroteknika.v6i2.224
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https://doi.org/10.1016/j.btre.2023.e00788.
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El-Darier, S. M., Metwally, A. F. K., Nasser, A. W., & Taha, H. M. (2021). Biointerference relationship between the macroalga Ulva lactuca and two green microalgae. The Egyptian Journal of Aquatic Research, 47(2), 163-169. https://doi.org/10.1016/j.ejar.2021.03.002.
Farasat, N., M., Riahi, H., & Koohdar, F. (2022). Study of morphological, anatomical characteristics and metabolic assay of some Ulva species (sea lettuce) in the coastal waters of the Persian Gulf, Iran. Biologica Reperta, 9 (3), 222-235. https://doi.org/10.52547/nbr.9.3.222
Fort, A., McHale, M., Cascella, K., Potin, P., Usadel, B., Guiry, M. D., & Sulpice, R. (2020). Foliose Ulva species show considerable inter‐specific genetic diversity, low intra‐specific genetic variation, and the rare occurrence of inter‐specific hybrids in the wild. Journal of phycology, 57(1), 219-233. https://doi.org/10.1111/jpy.13079
González, A., Osorio, H., Romero, S., Méndez, P., Sepúlveda, M., Laporte, D., & Moenne, A. (2022). Transcriptomic analyses reveal increased expression of dioxygenases, monooxygenases, and other metabolizing enzymes involved in anthracene degradation in the marine alga Ulva lactuca. Frontiers in Plant Science, 13, 955601. https://doi.org/10.3389/fpls.2022.955601
Hayati, R., Basyuni, M., & Chalil, D. (2020). Genetic diversity, sequence and bioinformatic analysis of Ganoderma boninense isolates. International Journal of Agriculture & Biology, 23(4), 763-770. https://www.researchgate.net/publication/339310608_Genetic_Diversity_Sequence_and_Bioinformatic_Analysis_of_Ganoderma_boninense_Isolates
Hughey, J. R., & Gabrielson, P. W. (2022). DNA analysis of the lectotype specimen of Ulva nematoidea Bory (Ulvaceae, Chlorophyta) determines its synonymy with Ulva lactuca L. Cryptogamie, Algologie, 43(7), 117-124. https://doi.org/10.5252/cryptogamie-algologie2022v43a7
Ismail, M. M., & Mohamed, E. S. (2017). Differentiation between some Ulva spp. by morphological, genetic and biochemical analyses. Frontiers, 21(3), 360-367. https://doi.org/10.18699/VJ17.253
Kong, S., Chen, Z., Zhao, F., Liu, Q., & Li, J. (2023). Cultivation of Ulva lactuca changes bacterial community structure and abundances of nitrogen cycling functional genes in an IMTA system. Retrieved from https://doi.org/10.21203/rs.3.rs-2801471/v1.
Lagourgue, L., Gobin, S., Brisset, M., Vandenberghe, S., Jauffrais, T., & Payri, C. E. (2022). Ten new species of Ulva (Ulvophyceae, Chlorophyta) discovered in New Caledonia: Genetic and morphological diversity, and bloom potential. European J. Phycology, 57 (4), 458-478. https://doi.org/10.1080/09670262.2022.2027023
Lenama, E., Bullu, N. I., & Kase, A. G. (2019). Struktur momunitas makroalga di perairan pantai Palibo Kecamatan Kabola Kabupaten Alor. J. Pend. dan Sains Biol. 2 73-84. Doi : 10.33323/indigenous.v2i2.32
Liu, J., Zhuang, M., Zhao, L., Liu, Y., Wen, Q., Fu, M., & He, P. (2020). Taxonomy and Genetic Diversity of Amphipods Living on Ulva lactuca L. from Gouqi Coast, China. Pacific Science, 74(2), 137-146. https://doi.org/10.2984/74.2.3
Mahendran, S., & Saravanan, S. (2017). Molecular taxonomy of green seaweeds Ulva lactuca and Caulerpa taxifolia through phylogenetic analysis. Indian Journal of Geo-Marine Sciences (IJMS), 46(02), 414-419. https://www.researchgate.net/publication/339934580
Malta, E. J., Draisma, S. G., & Kamermans, P. (1999). Free-floating Ulva in the southwest Netherlands: species or morphotypes? A morphological, molecular and ecological comparison. European journal of phycology, 34(5), 443-454. https://doi.org/10.1017/S0967026299002474
Mohan, E. H., Madhusudan, S., & Baskaran, R. (2023). The sea lettuce Ulva sensu lato: Future food with health-promoting bioactives. Algal Research, 103069.
https://doi.org/10.1016/j.algal.2023.103069
Mrowicki, R. J., & Brodie, J. (2023). The first record of a non-native seaweed from South Georgia and confirmation of its establishment in the Falkland Islands: Ulva fenestrata Postels & Ruprecht. Polar Biology, 46, 489-496. https://doi.org/10.1007/s00300-023-03136-6
O’kelly, C. J., Kurihara, A., Shipley, T. C., & Sherwood, A. R. (2010). Molecular Assessment of Ulva Spp. (Ulvophyceae, Chlorophyta) In The Hawaiian Islands 1. Journal of phycology, 46(4), 728-735. https://doi.org/10.1111/j.1529-8817.2010.00860.x
Purba, A., Hayati, R., Putri, L. A., Chalil, D., Syahputra, I., & Basyuni, M. (2020). Genetic diversity and structure of Ganoderma boninense isolates from oil palm and other plantation crops. Biodiversitas Journal of Biological Diversity, 21(2), 451-456. https://doi.org/10.13057/biodiv/d210204
Ramadhan, N., Martinsyah, R. H., Muhsanati, M., Obel, O., & Dwipa, I. (2023). Review Artikel: Keanekaragaman Hanjeli (Coix lacrimajobi L.) di Sumatera Barat. Agroteknika, 6(1), 57-69. https://doi.org/10.55043/agroteknika.v6i1.193
Ramesh, C. H., Prasastha, V. R., Shunmugaraj, T., Karthick, P., Mohanraju, R., Koushik, S., & Murthy, M. V. R. (2023). Diversity and impacts of macroalgae and cyanobacteria on multi-stressed coral reefs in the Gulf of Mannar Marine Biosphere Reserve. Marine Environmental Research, 106161. https://doi.org/10.1016/j.marenvres.2023.106161
Sari, M. (2016). Jenis keanekaragaman makroalgae di perairan pantai Ulee Paya Pulo Breuh sebagai penunjang mata kuliah botani tumbuhan rendah [Thesis]. 79.
Sari, V. K., & Restanto, D. P. (2022). Review Artikel: Metode Ekstraksi DNA Genom untuk Tanaman Tinggi Kandungan Polisakarida dan Metabolit Sekunder. Agroteknika, 5(2), 118-129. https://doi.org/10.55043/agroteknika.v5i2.155
Simon, C., McHale, M., & Sulpice, R. (2022). Applications of Ulva biomass and strategies to improve its yield and composition: A perspective for Ulva aquaculture. Biology, 11(11), 1593. https://doi.org/10.3390/biology11111593
Steinhagen, S., Hoffmann, S., Pavia, H., & Toth, G. B. (2023). Molecular identification of the ubiquitous green algae Ulva reveals high biodiversity, crypticity, and invasive species in the Atlantic-Baltic Sea region. Algal Research, 73, 103132. https://doi.org/10.1016/j.algal.2023.103132
Thanh, T. T. T., Quach, T. M. T., Nguyen, T. N., Luong, D. V., Bui, M. L., & van Tran, T. T. (2016). Structure and cytotoxic activity of ulvan extracted from green seaweed Ulva lactuca. International Journal of Biological Macromolecules, 93, 695-702. https://doi.org/10.1016/j.ijbiomac.2016.09.040
Tran, L. A. T., Leliaert, F., Vieira, C., Tran, T. V., Nguyen, T. V., Dam, T. D., & De Clerck, O. (2023). Molecular assessment of Ulva (Ulvales, Chlorophyta) diversity in Vietnam including the new species U. vietnamensis. Phycological Research, 71(1), 13-24. https://doi.org/10.1111/pre.12507
Tran, L. A. T., Vieira, C., Steinhagen, S., Maggs, C. A., Hiraoka, M., & Leliaert, F. (2022). An appraisal of Ulva (Ulvophyceae, Chlorophyta) taxonomy. J. Applied Phycol. 34 5 2689-2703. DOI: 10.1007/s10811-022-02815-x
Valério, F, A., Santana, L. R., Motta, N. G., Passos, L. F., Wolke, S., Mansilla, A., & Carreno, N. L. V. (2023). Extraction of fatty acids and cellulose from the biomass of algae Durvillaea antarctica and Ulva lactuca: An alternative for biorefineries. Algal Research, 71, 103084. https://doi.org/10.1016/j.algal.2023.103084
Ventura, M. R., & Castañón, J. I. R. (1998). The nutritive value of seaweed (Ulva lactuca) for goats. Small Ruminant Research, 29(3), 325-327.
https://doi.org/10.1016/S0921-4488(97)00134-X
Woolcott, G. W., & King, R. J. (1999). Ulva and Enteromorpha (Ulvales, Ulvophyceae, Chlorophyta) in eastern Australia: comparison of morphological features and analyses of nuclear rDNA sequence data. Australian Systematic Botany, 12(5), 709-725. https://doi.org/10.1071/SB97034
Bonanno, G., Veneziano, V., Raccuia, S. A., & Orlando-Bonaca, M. (2020). Seagrass Cymodocea nodosa and seaweed Ulva lactuca as tools for trace element biomonitoring. A comparative study. Marine Pollution Bulletin, 161, 111743.
https://doi.org/10.1016/j.marpolbul.2020.111743
Corredor, A. N. N., González, N. B. C., Acelas, M., & Castaño, D. M. (2023). Genome of the epiphytic bacterium Achromobacter denitrificans strain EPI24, isolated from a macroalga located in the Colombian Caribbean. Biotechnology Reports, 37, e00788.
https://doi.org/10.1016/j.btre.2023.e00788.
Dominguez, H., & Loret, E. P. (2019). Ulva lactuca, a Source of Troubles and Potential Riches. Marine drugs, 17(6), 357. https://doi.org/10.3390/md17060357
El-Darier, S. M., Metwally, A. F. K., Nasser, A. W., & Taha, H. M. (2021). Biointerference relationship between the macroalga Ulva lactuca and two green microalgae. The Egyptian Journal of Aquatic Research, 47(2), 163-169. https://doi.org/10.1016/j.ejar.2021.03.002.
Farasat, N., M., Riahi, H., & Koohdar, F. (2022). Study of morphological, anatomical characteristics and metabolic assay of some Ulva species (sea lettuce) in the coastal waters of the Persian Gulf, Iran. Biologica Reperta, 9 (3), 222-235. https://doi.org/10.52547/nbr.9.3.222
Fort, A., McHale, M., Cascella, K., Potin, P., Usadel, B., Guiry, M. D., & Sulpice, R. (2020). Foliose Ulva species show considerable inter‐specific genetic diversity, low intra‐specific genetic variation, and the rare occurrence of inter‐specific hybrids in the wild. Journal of phycology, 57(1), 219-233. https://doi.org/10.1111/jpy.13079
González, A., Osorio, H., Romero, S., Méndez, P., Sepúlveda, M., Laporte, D., & Moenne, A. (2022). Transcriptomic analyses reveal increased expression of dioxygenases, monooxygenases, and other metabolizing enzymes involved in anthracene degradation in the marine alga Ulva lactuca. Frontiers in Plant Science, 13, 955601. https://doi.org/10.3389/fpls.2022.955601
Hayati, R., Basyuni, M., & Chalil, D. (2020). Genetic diversity, sequence and bioinformatic analysis of Ganoderma boninense isolates. International Journal of Agriculture & Biology, 23(4), 763-770. https://www.researchgate.net/publication/339310608_Genetic_Diversity_Sequence_and_Bioinformatic_Analysis_of_Ganoderma_boninense_Isolates
Hughey, J. R., & Gabrielson, P. W. (2022). DNA analysis of the lectotype specimen of Ulva nematoidea Bory (Ulvaceae, Chlorophyta) determines its synonymy with Ulva lactuca L. Cryptogamie, Algologie, 43(7), 117-124. https://doi.org/10.5252/cryptogamie-algologie2022v43a7
Ismail, M. M., & Mohamed, E. S. (2017). Differentiation between some Ulva spp. by morphological, genetic and biochemical analyses. Frontiers, 21(3), 360-367. https://doi.org/10.18699/VJ17.253
Kong, S., Chen, Z., Zhao, F., Liu, Q., & Li, J. (2023). Cultivation of Ulva lactuca changes bacterial community structure and abundances of nitrogen cycling functional genes in an IMTA system. Retrieved from https://doi.org/10.21203/rs.3.rs-2801471/v1.
Lagourgue, L., Gobin, S., Brisset, M., Vandenberghe, S., Jauffrais, T., & Payri, C. E. (2022). Ten new species of Ulva (Ulvophyceae, Chlorophyta) discovered in New Caledonia: Genetic and morphological diversity, and bloom potential. European J. Phycology, 57 (4), 458-478. https://doi.org/10.1080/09670262.2022.2027023
Lenama, E., Bullu, N. I., & Kase, A. G. (2019). Struktur momunitas makroalga di perairan pantai Palibo Kecamatan Kabola Kabupaten Alor. J. Pend. dan Sains Biol. 2 73-84. Doi : 10.33323/indigenous.v2i2.32
Liu, J., Zhuang, M., Zhao, L., Liu, Y., Wen, Q., Fu, M., & He, P. (2020). Taxonomy and Genetic Diversity of Amphipods Living on Ulva lactuca L. from Gouqi Coast, China. Pacific Science, 74(2), 137-146. https://doi.org/10.2984/74.2.3
Mahendran, S., & Saravanan, S. (2017). Molecular taxonomy of green seaweeds Ulva lactuca and Caulerpa taxifolia through phylogenetic analysis. Indian Journal of Geo-Marine Sciences (IJMS), 46(02), 414-419. https://www.researchgate.net/publication/339934580
Malta, E. J., Draisma, S. G., & Kamermans, P. (1999). Free-floating Ulva in the southwest Netherlands: species or morphotypes? A morphological, molecular and ecological comparison. European journal of phycology, 34(5), 443-454. https://doi.org/10.1017/S0967026299002474
Mohan, E. H., Madhusudan, S., & Baskaran, R. (2023). The sea lettuce Ulva sensu lato: Future food with health-promoting bioactives. Algal Research, 103069.
https://doi.org/10.1016/j.algal.2023.103069
Mrowicki, R. J., & Brodie, J. (2023). The first record of a non-native seaweed from South Georgia and confirmation of its establishment in the Falkland Islands: Ulva fenestrata Postels & Ruprecht. Polar Biology, 46, 489-496. https://doi.org/10.1007/s00300-023-03136-6
O’kelly, C. J., Kurihara, A., Shipley, T. C., & Sherwood, A. R. (2010). Molecular Assessment of Ulva Spp. (Ulvophyceae, Chlorophyta) In The Hawaiian Islands 1. Journal of phycology, 46(4), 728-735. https://doi.org/10.1111/j.1529-8817.2010.00860.x
Purba, A., Hayati, R., Putri, L. A., Chalil, D., Syahputra, I., & Basyuni, M. (2020). Genetic diversity and structure of Ganoderma boninense isolates from oil palm and other plantation crops. Biodiversitas Journal of Biological Diversity, 21(2), 451-456. https://doi.org/10.13057/biodiv/d210204
Ramadhan, N., Martinsyah, R. H., Muhsanati, M., Obel, O., & Dwipa, I. (2023). Review Artikel: Keanekaragaman Hanjeli (Coix lacrimajobi L.) di Sumatera Barat. Agroteknika, 6(1), 57-69. https://doi.org/10.55043/agroteknika.v6i1.193
Ramesh, C. H., Prasastha, V. R., Shunmugaraj, T., Karthick, P., Mohanraju, R., Koushik, S., & Murthy, M. V. R. (2023). Diversity and impacts of macroalgae and cyanobacteria on multi-stressed coral reefs in the Gulf of Mannar Marine Biosphere Reserve. Marine Environmental Research, 106161. https://doi.org/10.1016/j.marenvres.2023.106161
Sari, M. (2016). Jenis keanekaragaman makroalgae di perairan pantai Ulee Paya Pulo Breuh sebagai penunjang mata kuliah botani tumbuhan rendah [Thesis]. 79.
Sari, V. K., & Restanto, D. P. (2022). Review Artikel: Metode Ekstraksi DNA Genom untuk Tanaman Tinggi Kandungan Polisakarida dan Metabolit Sekunder. Agroteknika, 5(2), 118-129. https://doi.org/10.55043/agroteknika.v5i2.155
Simon, C., McHale, M., & Sulpice, R. (2022). Applications of Ulva biomass and strategies to improve its yield and composition: A perspective for Ulva aquaculture. Biology, 11(11), 1593. https://doi.org/10.3390/biology11111593
Steinhagen, S., Hoffmann, S., Pavia, H., & Toth, G. B. (2023). Molecular identification of the ubiquitous green algae Ulva reveals high biodiversity, crypticity, and invasive species in the Atlantic-Baltic Sea region. Algal Research, 73, 103132. https://doi.org/10.1016/j.algal.2023.103132
Thanh, T. T. T., Quach, T. M. T., Nguyen, T. N., Luong, D. V., Bui, M. L., & van Tran, T. T. (2016). Structure and cytotoxic activity of ulvan extracted from green seaweed Ulva lactuca. International Journal of Biological Macromolecules, 93, 695-702. https://doi.org/10.1016/j.ijbiomac.2016.09.040
Tran, L. A. T., Leliaert, F., Vieira, C., Tran, T. V., Nguyen, T. V., Dam, T. D., & De Clerck, O. (2023). Molecular assessment of Ulva (Ulvales, Chlorophyta) diversity in Vietnam including the new species U. vietnamensis. Phycological Research, 71(1), 13-24. https://doi.org/10.1111/pre.12507
Tran, L. A. T., Vieira, C., Steinhagen, S., Maggs, C. A., Hiraoka, M., & Leliaert, F. (2022). An appraisal of Ulva (Ulvophyceae, Chlorophyta) taxonomy. J. Applied Phycol. 34 5 2689-2703. DOI: 10.1007/s10811-022-02815-x
Valério, F, A., Santana, L. R., Motta, N. G., Passos, L. F., Wolke, S., Mansilla, A., & Carreno, N. L. V. (2023). Extraction of fatty acids and cellulose from the biomass of algae Durvillaea antarctica and Ulva lactuca: An alternative for biorefineries. Algal Research, 71, 103084. https://doi.org/10.1016/j.algal.2023.103084
Ventura, M. R., & Castañón, J. I. R. (1998). The nutritive value of seaweed (Ulva lactuca) for goats. Small Ruminant Research, 29(3), 325-327.
https://doi.org/10.1016/S0921-4488(97)00134-X
Woolcott, G. W., & King, R. J. (1999). Ulva and Enteromorpha (Ulvales, Ulvophyceae, Chlorophyta) in eastern Australia: comparison of morphological features and analyses of nuclear rDNA sequence data. Australian Systematic Botany, 12(5), 709-725. https://doi.org/10.1071/SB97034